EMPIAR-10090
Nucleotide-Driven Triple-State Remodeling of the AAA-ATPase Channel in the Activated Human 26S Proteasome [multiple data sets in MRC and MRCS formats]
Publication:

Structural mechanism for nucleotide-driven remodeling of the AAA-ATPase unfoldase in the activated human 26S proteasome

Zhu Y, Wang WL, Yu D, Ouyang Q, Lu Y, Mao Y

Nature communications 9 (2018)

PMID: 29636472

Deposited:
2017-04-09
Released:
2018-07-18
Last modified:
2018-07-18
Imageset size:
2.83 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - single frame
  • picked particles - multiframe - processed
1. Motion-corrected single frame micrographs of ATP-gS-bound human proteasome
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
8463
Image size:
(7420, 7676)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.75, 0.75)
Details:
Each micrograph is averaged from drift-corrected 30 frames with an accumulated dose of 30 electrons/anstrom^2.
Files:
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2. Single-particle stacks of classified conformations
Category:
picked particles - multiframe - processed
Image format:
MRCS
No. of images or tilt series:
502384
Image size:
(560, 560)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.75, 0.75)
Details:
The single-particle datasets have been classified into six classes: SA, SB, SC, SD1, SD2, SD3. The SD20S is a combined data set of SD1, SD2 and SD3 to focus refinement on the 20S CP component of the human proteasome in an open-gate state.
Files:
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