EMPIAR-10077 - The pathway to GTPase activation of elongation factor SelB on the ribosome [multiple data sets in MRC format]

EMPIAR-10077

The pathway to GTPase activation of elongation factor SelB on the ribosome [multiple data sets in MRC format]

Publication:

The pathway to GTPase activation of elongation factor SelB on the ribosome

Neumann P, Bock LV, Maracci C, Wang Z, Paleskava A, Konevega AL, Schroeder GF, Grubmueller H, Ficner R, Rodnina MV, Stark H

Nature (2016)

PMID: 27842381

DOI: 10.1038/nature20560

Related EMDB entry:

EMD-4126 EMD-4121 EMD-4122 EMD-4125 EMD-4124 EMD-4123

Deposited:

2016-11-01

Released:

2016-11-24

Last modified:

2016-11-24

Imageset size:

1.03 TB

Imageset DOI:

10.6019/EMPIAR-10077

Experimental metadata:

Download xml json

Contains:

micrographs - single frame
picked particles - multiframe - processed

Image Sets

1. Part 1 - Unprocessed cryo-EM micrographs of E. Coli 70S-SelB-GDPNP-Sec-tRNASec-fMet-tRNAfMet-SECIS mRNA complexes

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

6147

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.16, 1.16)

Details:

---

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2. Part 2 - Unprocessed cryo-EM micrographs of E. Coli 70S-SelB-GDPNP-Sec-tRNASec-fMet-tRNAfMet-SECIS mRNA complex

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

6534

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.16, 1.16)

Details:

---

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. ribosomeSelB_particles.mrcs - CTF corrected single particle images of E. Coli 70S-SelB-GDPNP-Sec-tRNASec-fMet-tRNAfMet-SECIS mRNA complex

Category:

picked particles - multiframe - processed

Image format:

MRC

No. of images or tilt series:

969526

Image size:

(272, 272)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.16, 1.16)

Details:

Particle image stack "ribosomeSelB_particles.mrcs":
CTF corrected particles images (not normalized; total 969.526 particles)

Star-files for particles corresponding to distinct states in "ribosomeSelB_particles.mrcs":
1. "particles_IC_state.star": particles of initial complex, E. coli ribosome 70S-fMet- tRNAfmet-SECIS mRNA complex (IC)
2. "particles_IB_state.star": particles of initial binding state of E. coli ribosome 70S-SelB-GDPNP-Phe-tRNAPhe-fMet-tRNAfmet-SECIS mRNA complex (IB)
3. "particles_CR_state.star": particles of codon reading state of E. coli ribosome 70S-SelB-GDPNP-Phe-tRNAPhe-fMet-tRNAfmet-SECIS mRNA complex (CR)
4. "particles_GA_state.star": particles of GTPase activated state of E. coli ribosome 70S-SelB-GDPNP-Phe-tRNAPhe-fMet-tRNAfmet-SECIS mRNA complex (GA)
5. "particles_C_state.star": particles of classical pre-translocation state of E. coli ribosome 70S-Phe-tRNAPhe-fMet-tRNAfmet-SECIS mRNA complex (C)
6. "particles_H_state.star": particles of hybrid pre-translocation state of E. coli ribosome 70S-Phe-tRNAPhe-fMet-tRNAfmet-SECIS mRNA complex (H)

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