2.2 Å resolution cryo-EM structure of β-galactosidase in complex with a cell-permeant inhibitor

Bartesaghi A, Merk A, Banerjee S, Matthies D, Wu X, Milne JL, Subramaniam S

Science (New York, N.Y.) 348 (2015) 1147-1151

Movies (Movies/*.mrc) in this entry were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

Compression was carried out by separating (“un-normalizing”) the gain.
The details of the method are described in
https://relion.readthedocs.io/en/release-3.1/Reference/MovieCompression.html#gain-normalised-movies.

The movies were grouped into three sets (gainset1 to gainset3), which differ by the gain.
Please apply the gain reference (e.g. gainset1/gain1.mrc) in the respective folder during processing.

gainset1:   19
gainset2: 1058
gainset3:  462
---------------------
total:    1539 movies

Original size: 13,017,514,143 kilobytes
Compressed size:  319,467,241 kilobytes