EMPIAR-10061 - 2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor [multiple data sets in MRC format]

EMPIAR-10061

2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor [multiple data sets in MRC format]

Publication:

2.2 Å resolution cryo-EM structure of β-galactosidase in complex with a cell-permeant inhibitor

Bartesaghi A, Merk A, Banerjee S, Matthies D, Wu X, Milne JL, Subramaniam S

Science (New York, N.Y.) 348 (2015) 1147-1151

PMID: 25953817

DOI: 10.1126/science.aab1576

Related EMDB entry:

EMD-2984

Deposited:

2016-04-11

Released:

2016-04-15

Last modified:

2019-08-24

Imageset size:

677.76 GB

Imageset DOI:

10.6019/EMPIAR-10061

Experimental metadata:

Download xml json

Version history:

2016-05-13

REL

Added the particle coordinates in EMD-2984_boxes.tbz archive.

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

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Contains:

micrographs - single frame
micrographs - multiframe

Image Sets

1. Averages of aligned movie frames

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

1539

Image size:

(7676, 7420)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.3185, 0.3185)

Details:

Micrographs are the average of frames 1-38 after alignment. The particle coordinates are located in EMD-2984_boxes.tbz archive. There is one file for each micrograph containing the coordinates stored in EMAN's box format.

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2. Raw movie frames

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

1539

Image size:

(7676, 7420)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.3185, 0.3185)

Details:

---

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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