EMPIAR-10061
2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor [multiple data sets in MRC format]
Publication:

2.2 Å resolution cryo-EM structure of β-galactosidase in complex with a cell-permeant inhibitor

Bartesaghi A, Merk A, Banerjee S, Matthies D, Wu X, Milne JL, Subramaniam S

Science (New York, N.Y.) 348 (2015) 1147-1151

PMID: 25953817

Related EMDB entry:
Deposited:
2016-04-11
Released:
2016-04-15
Last modified:
2019-08-24
Imageset size:
631.21 GB
Imageset DOI:
Experimental metadata:
Download xml json
Version history:
1
2016-05-13
REL
Added the particle coordinates in EMD-2984_boxes.tbz archive.

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies (Movies/*.mrc) in this entry were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

Compression was carried out by separating (“un-normalizing”) the gain.
The details of the method are described in
https://relion.readthedocs.io/en/release-3.1/Reference/MovieCompression.html#gain-normalised-movies.

The movies were grouped into three sets (gainset1 to gainset3), which differ by the gain.
Please apply the gain reference (e.g. gainset1/gain1.mrc) in the respective folder during processing.

gainset1:   19
gainset2: 1058
gainset3:  462
---------------------
total:    1539 movies

Original size: 13,017,514,143 kilobytes
Compressed size:  319,467,241 kilobytes
Contains:
  • micrographs - single frame
  • micrographs - multiframe
1. Averages of aligned movie frames
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
1539
Image size:
(7676, 7420)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.3185, 0.3185)
Details:
Micrographs are the average of frames 1-38 after alignment. The particle coordinates are located in EMD-2984_boxes.tbz archive. There is one file for each micrograph containing the coordinates stored in EMAN's box format.
Files:
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2. Raw movie frames
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
1539
Image size:
(7676, 7420)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.3185, 0.3185)
Details:
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Files:
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Files:
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