EMPIAR-10016 - Yeast 80S Ribosome - tRNA- Kozak mRNA complexes, Frealign Input Particle Stack [stack of 86866 particles in MRC format]

EMPIAR-10016

Yeast 80S Ribosome - tRNA- Kozak mRNA complexes, Frealign Input Particle Stack [stack of 86866 particles in MRC format]

Publication:

Structures of Yeast 80S Ribosome-tRNA Complexes in the Rotated and Nonrotated Conformations.

Svidritskiy E, Brilot AF, Koh CS, Grigorieff N, Korostelev AA

Structure 22 (2014) 1210-1218

PMID: 25043550

DOI: doi:10.1016/j.str.2014.06.003

Related EMDB entry:

EMD-5977 EMD-5976

Deposited:

2014-06-04

Released:

2014-11-06

Last modified:

2016-03-17

Imageset size:

42.01 GB

Imageset DOI:

10.6019/EMPIAR-10016

Experimental metadata:

Download xml json

Version history:

2015-10-28

REL

Restructured the entry directory to have "data" and ".private".

2016-03-17

REL

Added Frealign parameter files that correspond to the classes of the deposited data.

Contains:

picked particles - multiframe - processed

Image Sets

1. Frealign input particle stack

Category:

picked particles - multiframe - processed

Image format:

MRC

No. of images or tilt series:

86866

Image size:

(360, 360)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.05, 1.05)

Details:

Processing of raw images: Normalized in Imagic (normvar, whole image average=0, sigma=10). Originally the data was classified into five classes. The five Frealign parameter files that correspond to these classes can be found in "parfiles" directory.

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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