EMPIAR-10013
Structure of β-galactosidase at 3.2-Å resolution obtained by cryo-electron microscopy [multiple data sets in MRC and DM4 formats]
Publication:

Structure of beta-galactosidase at 3.2-A resolution obtained by cryo-electron microscopy

Bartesaghi A, Matthies D, Banerjee S, Merk A, Subramaniam S

Pnas 111 (2014) 11709-11714

PMID: 25071206

Related EMDB entry:
Deposited:
2014-07-23
Released:
2014-08-07
Last modified:
2014-08-07
Imageset size:
442.52 GB
Imageset DOI:
Experimental metadata:
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Version history:
1
2015-07-01
REL
Directory structure reorganized. Entry has now full set of micrographs including frame alignment shifts, particle locations and defoci.
Contains:
  • micrographs - multiframe
1. Full set of multi-frame micrographs
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
509
Image size:
(7420, 7676)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.6375, 0.6375)
Details:
Full set of multiframe micrographs related to the dataset:10012. Related PDB: 3J7H.
Please note that the number of frames varies between [5, 10, 25 and 38]!
Although the image files are in MRC format, they have been compressed to save space. They can be uncompresssed using for example: for f in *.tbz; do tar -xjf $f -C mrc/ ; done
Image files are numbered: BGal_000000_frames.tbz to BGal_000508_frames.tbz
Frame alignment shifts in IMOD's transform format: BGal_000000.xf to BGal_000508.xf
Picked particle locations in EMAN's boxer format: BGal_000000.box to BGal_000508.box
Defocus per image in Angstroms: BGal_defocus.txt
Additional details:
Cs : 2.7
Anisotropic magnification (if known) ? : N/A
frame rate : 2.5 to .33 frames/s
dose per frame : 1.2 to 9 e-/A^2
alignment method/software : 10.1073/pnas.1402809111
which frame used for alignment reference: middle frame
particle selection software : 10.1073/pnas.1402809111
Files:
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2. Sample set of multi-frame micrographs
Category:
micrographs - multiframe
Image format:
DM4
No. of images or tilt series:
5
Image size:
(7420, 7676)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.6375, 0.6375)
Details:
Selection of 5 multiframe micrographs related to the dataset:10012. Related PDB: 3J7H.
Files:
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Files:
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