EMPIAR-12641
Pre-catalytic complex micrographs [935 multi-frame micrographs composed of 40 frames each in MRC format]
Publication:

Structure of the TnsB transposase-DNA complex of type V-K CRISPR-associated transposon

Tenjo-Castaño F, Sofos N, López-Méndez B, Stutzke LS, Fuglsang A, Stella S, Montoya G

Nature communications 13 (2022)

PMID: 36184667

Related EMDB entry:
Deposited:
2025-03-18
Released:
2025-04-23
Last modified:
2025-04-23
Imageset size:
604.74 GB
Imageset DOI:
Experimental metadata:
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Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (FoilHole*Fractions.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

Original size:   1,225,524,135 kilobytes
Compressed size:   586,254,134 kilobytes
Contains:
  • micrographs - multiframe
1. TnsB pre-catalytic complex micrographs
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
935
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(0.98, 0.98)
Details:
Raw pixel size: 0.98 Å
Accelerating Voltage: 200 kV
Spherical Aberration: 2.7 mm
Total exposure dose: 39 e/Å^2
Frames: 40
Files:
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Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)
Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)
Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)
Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)