EMPIAR-12629
Cryo-EM of SARS-CoV-2 nsp13-replication-transcription complex (nsp13-RTC) (no detergent) [4358 multi-frame micrographs composed of 50 frames each in TIFF format]
Publication:

Structural basis for helicase-polymerase coupling in the SARS-CoV-2 replication-transcription complex

Chen J, Malone B, Llwewllyn E, Grasso M, Shelton PMM, Olinares PDB, Maruthi K, ENG ET, Vatandaslar H, Chait BT, Kapoor TM, Darst SA, Campbell EA

Cell 182 (2020) 1560-1573

PMID: 32783916

Related EMDB entry:
Deposited:
2025-02-25
Released:
2025-04-24
Last modified:
2025-04-24
Imageset size:
3.54 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. unaligned raw movies
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
4358
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.1, 1.1)
Details:
FEI Titan Krios + Gatan K3, counting mode, BioQuantum energy filter 20 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 24.7 e-/ A^2/s, frame time 0.05 s (50 frames) with total exposure of 2.5 s, total dose of 64 e-/A^2; pixel size 1.1 A/px(detector); defocus range -0.8 to -2.5 um; for gain reference, see data/rawdata/references/reference_list.txt.
Files:
Loading...
Kim K, Li H, Clarke OB. (2025)
Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)
Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)
Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)
Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)
Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)
Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)
Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)
Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)
Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)