EMPIAR-12595 - Cryo-EM of RNAP post-terminal complexes bound to RapA+ADP+AlF3 on negatively supercoiled DNA [multiple data sets in TIFF format]

EMPIAR-12595

Cryo-EM of RNAP post-terminal complexes bound to RapA+ADP+AlF3 on negatively supercoiled DNA [multiple data sets in TIFF format]

Publication:

RapA opens the RNA polymerase clamp to disrupt post-termination complexes and prevent cytotoxic R-loop formation

Brewer JJ, Inlow K, Mooney RA, Bosch B, Olinares PDB, Marcelino LP, Chait BT, Landick R, Gelles J, Campbell EA, Darst SA

Nature structural & molecular biology (2025)

PMID: 39779919

DOI: 10.1038/s41594-024-01447-8

Related EMDB entry:

EMD-40943

Deposited:

2025-02-28

Released:

2025-04-09

Last modified:

2025-04-09

Imageset size:

1.67 TB

Imageset DOI:

10.6019/EMPIAR-12595

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned raw movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

20770

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.076, 1.076)

Details:

FEI Titan Krios + Gatan K3, counting mode, BioQuantum energy filter 20 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 28.40 e-/ A^2/s, frame time 0.05 s (38 frames) with total exposure of 1.90 s, total dose of 53.95 e-/A^2, pixel size 1.076 A/px; defocus range -0.9 to -2.5 um; gain reference is 22jun18a_18113825_01_4092x5760_norm_0.mrc (see references folder).

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2. Unaligned raw movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

20770

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.844, 0.844)

Details:

FEI Titan Krios + Gatan K3, counting mode, BioQuantum energy filter 20 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 28.40 e-/ A^2/s, frame time 0.05 s (38 frames) with total exposure of 1.90 s, total dose of 53.95 e-/A^2, pixel size 0.844 A/px(detector); pixel size 0.422 A/px(image); defocus range -0.9 to -2.5 um; gain reference is 22jun18a_18113825_01_4092x5760_norm_0.mrc (see references folder).

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

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Citations

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Wang X, Li F, Zhang C, Tang S, Jia Z, Hu B, Zhang F, Wan X. (2026)

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Cheng Y, He B, Zeng Q, Zhang F, Han R. (2026)

CryoMV: Structure-Prior-Guided Modeling and Real-Particle Validation of Continuous Conformational Transitions in Cryo-EM

Li F, Dong H, Tang S, Wang X, Zhang C, Ji C, Han R, Zhang F, Wan X. (2026)

Structural identification of the RY12 domain of RyR1 as an ADP sensor and the target of the malignant hyperthermia therapeutic dantrolene.

Kim K, Li H, Yuan Q, Melville Z, Zalk R, des Georges A, Frank J, Hendrickson WA, Marks AR, Clarke OB. (2026)

Structural heterogeneity of CTE Type I tau filaments with an interface-shifted polymorph.

Watanabe R, Lee EB. (2026)

Post-acquisition super resolution for cryo-electron microscopy.

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Watanabe R, Lee EB. (2026)

Agentic cryo-EM structure determination with CryoPilot

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Ligand-induced activation of RyR1 in native membranes.

Mikirtumov V, Golusik S, Huo R, Sprink T, Balyschew N, Yang W, Diebolder C, Yuan S, Kotecha A, Kudryashev M. (2026)

QwenCryoMarker: a universal post-processing framework for contamination-aware particle cleaning.

Sun Y, Zhao J, Xu N, Wang L, Ding W, Li M. (2026)

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