EMPIAR-12445 - Cryo electron microscopy of Medusavirus medusae nucleosome-like particle [multiple data sets in EER and MRC formats]

EMPIAR-12445

Cryo electron microscopy of Medusavirus medusae nucleosome-like particle [multiple data sets in EER and MRC formats]

Publication:

Characterization of Medusavirus encoded histones reveals nucleosome-like structures and a unique linker histone

Toner CM, Hoitsma NM, Weerawarana S, Luger K

Nature communications 15 (2024)

PMID: 39443461

DOI: 10.1038/s41467-024-53364-5

Related EMDB entry:

EMD-45981 EMD-42053

Deposited:

2024-09-17

Released:

2024-12-03

Last modified:

2024-12-03

Imageset size:

6.65 TB

Imageset DOI:

10.6019/EMPIAR-12445

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame
picked particles - multiframe - processed
reconstructed volumes

Image Sets

1. Unaligned multi-frame micrographs of the medusavirus core particle in EER format

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

8534

Image size:

(None, None)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.97, 0.97)

Details:

Raw EER files from Falcon 4 detector, acquired with EPU and a total dose of 50 e-/Å2

Files:

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2. Motion-corrected and dose-weighted micrographs

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

8534

Image size:

(None, None)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.97, 0.97)

Details:

Motion-corrected and dose-weighted micrographs

Files:

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Archives and downloads the selected files into an uncompressed zip file.

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3. Final particles used for reconstruction

Category:

picked particles - multiframe - processed

Image format:

MRC

No. of images or tilt series:

159734

Image size:

(None, None)

Pixel type:

16 BIT FLOAT

Pixel spacing:

(0.97, 0.97)

Details:

Final particles used for reconstruction

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

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4. CryoSPARC job of the final 3D reconstruction

Category:

reconstructed volumes

Image format:

MRC

No. of images or tilt series:

159734

Image size:

(None, None)

Pixel type:

16 BIT FLOAT

Pixel spacing:

(None, None)

Details:

CryoSPARC job of the final 3D reconstruction

Files:

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Archives and downloads the selected files into an uncompressed zip file.

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5. Gain ref file

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

Image size:

(None, None)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.97, 0.97)

Details:

Gain ref file for motion correction

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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