EMPIAR-12443 - Single particle reconstruction of human apo TRPM8 channel in a closed state [multiple data sets in TIFF and MRCS formats]

EMPIAR-12443

Single particle reconstruction of human apo TRPM8 channel in a closed state [multiple data sets in TIFF and MRCS formats]

Publication:

Structure of human TRPM8 channel

Palchevskyi S, Czarnocki-Cieciura M, Vistoli G, Gervasoni S, Nowak E, Beccari AR, Nowotny M, Talarico C

Communications biology 6 (2023)

PMID: 37857704

DOI: 10.1038/s42003-023-05425-6

Related EMDB entry:

EMD-15981 EMD-15983 EMD-15982

Deposited:

2024-10-29

Released:

2025-02-03

Last modified:

2025-02-03

Imageset size:

3.69 TB

Imageset DOI:

10.6019/EMPIAR-12443

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
picked particles - single frame - processed

Image Sets

1. Unaligned multi-frame movies of human apo TRPM8 channel in a closed state

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7918

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.82, 0.82)

Details:

Gain-corrected, unaligned multi-frame movies collected with Krios G3i microscope + K3 detector (superresolution mode, binned 2x with EPU). Voltage: 300 kV; spherical aberration: 2.7; dose per frame: 1 e/A^2 (total dose: 41.42 e/A^2 fractionated over 40 frames). Defocus range (nominal): -2.7, -2.4, -2.1, -1.8, -1.5, -1.2, -0.9 µm. Objective aperture retracted, C2 aperture: 50 µm.

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2. Aligned particles of human apo TRPM8 channel in a closed state (consensus map)

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

110176

Image size:

(420, 420)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.82, 0.82)

Details:

Aligned particles with corresponding Relion-3 STAR file for the consensus TRPM8 reconstruction. Coordinates correspond to micrographs binned 2x during Motion correction.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Aligned particles of human apo TRPM8 channel in a closed state (focused pre-MHR + MHR1/2 map)

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

212851

Image size:

(192, 192)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.82, 0.82)

Details:

Aligned particles with corresponding Relion-3 STAR file for the focused TRPM8 reconstruction (pre-MHR + MHR1/2 map). Particles were signal-subtracted; coordinates correspond to micrographs binned 2x during Motion correction.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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