EMPIAR-12337 - CryoEM of E. coli RNA polymerase on lambda PR -5G to -5C promoter DNA [4437 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-12337

CryoEM of E. coli RNA polymerase on lambda PR -5G to -5C promoter DNA [4437 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

Structural origins of Escherichia coli RNA polymerase open promoter complex stability

Saecker RM, Chen J, Chiu CE, Malone B, Sotiris J, Ebrahim M, Yen LY, Eng ET, Darst SA

Proceedings of the National Academy of Sciences of the United States of America 118 (2021)

PMID: 34599106

DOI: 10.1073/pnas.2112877118

Related EMDB entry:

EMD-23895

Deposited:

2024-10-04

Released:

2024-12-05

Last modified:

2024-12-05

Imageset size:

1.81 TB

Imageset DOI:

10.6019/EMPIAR-12337

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned raw movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4437

Image size:

(7420, 7676)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.03, 1.03)

Details:

FEI Titan Krios + Gatan K2, super-resolution, energy filter 20 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 7.5 e-/ A^2/s; frame time 0.2 s (50 frames) with total exposure of 10 s; total dose 75 e-/A^2; pixel size 1.03 A/px(detector); pixel size 0.515 A/px(image); defocus range -1.1 to -2.4 um; gain reference is reference_fliprot90.mrc located in references folder.

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