EMPIAR-12337 - CryoEM of E. coli RNA polymerase on lambda PR -5G to -5C promoter DNA [4437 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-12337

CryoEM of E. coli RNA polymerase on lambda PR -5G to -5C promoter DNA [4437 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

Structural origins of Escherichia coli RNA polymerase open promoter complex stability

Saecker RM, Chen J, Chiu CE, Malone B, Sotiris J, Ebrahim M, Yen LY, Eng ET, Darst SA

Proceedings of the National Academy of Sciences of the United States of America 118 (2021)

PMID: 34599106

DOI: 10.1073/pnas.2112877118

Related EMDB entry:

EMD-23895

Deposited:

2024-10-04

Released:

2024-12-05

Last modified:

2024-12-05

Imageset size:

1.99 TB

Imageset DOI:

10.6019/EMPIAR-12337

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned raw movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4437

Image size:

(7420, 7676)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.03, 1.03)

Details:

FEI Titan Krios + Gatan K2, super-resolution, energy filter 20 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 7.5 e-/ A^2/s; frame time 0.2 s (50 frames) with total exposure of 10 s; total dose 75 e-/A^2; pixel size 1.03 A/px(detector); pixel size 0.515 A/px(image); defocus range -1.1 to -2.4 um; gain reference is reference_fliprot90.mrc located in references folder.

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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