EMPIAR-12317 - Micrographs for mouse alpha synuclein [multiple data sets in MRC format]

EMPIAR-12317

Micrographs for mouse alpha synuclein [multiple data sets in MRC format]

Publication:

Mouse α-synuclein fibrils are structurally and functionally distinct from human fibrils associated with Lewy body diseases

Sokratian A, Zhou Y, Tatli M, Burbidge KJ, Xu E, Viverette E, Donzelli S, Duda AM, Yuan Y, Li H, Strader S, Patel N, Shiell L, Malankhanova T, Chen O, Mazzulli JR, Perera L, Stahlberg H, Borgnia M, Bartesaghi A, Lashuel HA, West AB

Science advances 10 (2024)

PMID: 39485845

DOI: 10.1126/sciadv.adq3539

Related EMDB entry:

EMD-50023 EMD-42294

Deposited:

2024-04-03

Released:

2025-07-16

Last modified:

2025-07-16

Imageset size:

467.89 GB

Imageset DOI:

10.6019/EMPIAR-12317

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Aligned micrographs of mouse alpha-synuclein

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

5358

Image size:

(5760, 4092)

Pixel type:

16 BIT FLOAT

Pixel spacing:

(0.878, 0.878)

Details:

The data was collected using a Glacios 200 kV system equipped with a K3 camera. A total of 5358 micrographs were motion-corrected, with a 180-degree rotation gain reference was applied, utilizing RELION's implementation of MotionCor2 for alignment. Coordinates for manually picked particles are available in the ManualPick folder.

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2. Aligned micrographs of mouse alpha-synuclein dataset 2

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

2579

Image size:

(5760, 4092)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.08, 1.08)

Details:

The data was collected using a Titan Krios 300 kV system equipped with a K3 camera. Movie frames were gain-corrected, aligned, and dose-weighed using Relion 4.0 software. A total of 2579 micrographs from 2 sessions of data collection are used in the reconstruction. Coordinates for manually picked particles are available in the ManualPick folder.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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Citations

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Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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