EMPIAR-12289 - Multi-frame cryo-EM images of a 69 kDa KRAS:G12C-2H11.4DS Fab complex [5013 multi-frame micrographs composed of 119 frames each in TIFF format]

EMPIAR-12289

Multi-frame cryo-EM images of a 69 kDa KRAS:G12C-2H11.4DS Fab complex [5013 multi-frame micrographs composed of 119 frames each in TIFF format]

Publication:

Disulfide constrained Fabs overcome target size limitation for high-resolution single-particle cryo-EM

Kung JE, Johnson MC, Jao CC, Arthur CP, Tegunov D, Rohou A, Sudhamsu J

bioRxiv (2024)

DOI: 10.1101/2024.05.10.593593

Related EMDB entry:

EMD-43221

Deposited:

2024-05-16

Released:

2025-07-16

Last modified:

2025-07-16

Imageset size:

1.91 TB

Imageset DOI:

10.6019/EMPIAR-12289

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Multi-frame cryo-EM images of a 69 kDa KRAS:G12C-2H11.4DS Fab complex in tiff format

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5013

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.838, 0.838)

Details:

Multi-frame cryo-EM images of a 69 kDa KRAS:G12C-2H11.4DS Fab complex in tiff format.

Gain file: CountCDSRef_movie_77-1_000_Mar20_18.55.42_1-0.mrc
Pixel spacing: 0.838Å
Total dose per image: 68.277 electrons/Å²
Frames per image: 119
Objective aperture diameter: 100μm
C2 aperture diameter: 50μm
Gun lens setting: 4
Energy filter slit width: 20eV

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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