EMPIAR-12271 - Cryo electron tomography of Enterovirus 71-infected cells [1 tilt series in MRC format]

EMPIAR-12271

Cryo electron tomography of Enterovirus 71-infected cells [1 tilt series in MRC format]

Publication:

Endosome rupture enables enteroviruses to infect cells

Ishemgulova A, Mukhamedova L, Trebichalská Z, Rájecká V, Payne P, Šmerdová L, Moravcová J, Hrebík D, Buchta D, Škubník K, Füzik T, Vaňáčová Š, Nováček J, Plevka P

Communications Biology (---)

Related EMDB entry:

EMD-15710 EMD-15711

Deposited:

2024-09-05

Released:

2025-01-09

Last modified:

2025-01-09

Imageset size:

2.53 GB

Imageset DOI:

10.6019/EMPIAR-12271

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Cryo electron tomography of Enterovirus 71-infected cos-7 cells

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(None, None)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(5.532, 5.532)

Details:

An additional tomography of Enterovirus 71-infected cell. This one was used to depict the empty ruptured endosomes in the infected cell (Supplementary material Figure).
Tilt series:
- pixel width and height: 5.532 Å
- Number of tilts: 41
- number of columns, rows: 3838, 3710
- dose-symmetric tilt scheme, ± 50°, 2.5° tilt increment
- raw tilt file available
Tomograms:
- pixel width and height: 16.60 Å

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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