EMPIAR-12193 - FAST-EM array tomography data of rat pancreas prepared with rOTO protocol and stained with neodymium acetate [4805 micrographs in TIFF format]

EMPIAR-12193

FAST-EM array tomography data of rat pancreas prepared with rOTO protocol and stained with neodymium acetate [4805 micrographs in TIFF format]

Publication:

FAST-EM array tomography: a workflow for multibeam volume electron microscopy

Kievits AJ, Duinkerken BHP, Lane R, de Heus C, van Beijeren Bergen En Henegouwen D, Höppener T, Wolters AHG, Liv N, Giepmans BNG, Hoogenboom JP

Methods in microscopy 1 (2024) 49-64

PMID: 39119255

DOI: 10.1515/mim-2024-0005

Related EMDB entry:

Deposited:

2024-06-20

Released:

2024-11-19

Last modified:

2024-11-19

Imageset size:

514.96 GB

Imageset DOI:

10.6019/EMPIAR-12193

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Raw FAST-EM array tomography electron micrographs from wild-type MCF-7 cells stained with rOTO protocol and neodymium acetate

Category:

micrographs - single frame

Image format:

TIFF

No. of images or tilt series:

4805

Image size:

(6400, 6400)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(40.0, 40.0)

Details:

Raw serial-section (array tomography) data of MCF-7 cells acquired with FAST-EM, ordered per section. Each section directory (SXX) contains 10x10 images covering an area of approximately 240 x 240 microns, with 6.25% overlap between images. Images were acquired with 20 us dwell time.

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

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In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

CryoPROS: Correcting misalignment caused by preferred orientation using AI-generated auxiliary particles.

Zhang H, Zheng D, Wu Q, Yan N, Peng H, Hu Q, Peng Y, Yan Z, Shi Z, Bao C, Hu M. (2025)

Few-shot learning for non-vitrified ice segmentation.

Vivas-Lago A, Castaño-Díez D. (2025)

Automated removal of corrupted tilts in cryo-electron tomography.

Majtner T, Turoňová B. (2025)

Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with both 2:1 and 2:2 stoichiometries.

Felix J, De Munck S, Bazan JF, Savvides SN. (2025)

Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE.

Van CTS, Reboul CF, Caesar JJE, Meana-Pañeda R, Lountos GT, Deme JC, Bryant OJ, Johnson S, Piczak CT, Valkov E, Lea SM, Elmlund H. (2025)

Cryo-EM Structure Reconstruction by Gaussian Splatting: Pushing the Resolution to Extreme

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2025)

Molecular exaptation by the integrin αI domain.

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