EMPIAR-12188 - Cryo-EM reconstruction of the full-length H209N mutant E. coli transmembrane formate transporter FocA [2128 multi-frame micrographs composed of 30 frames each in MRC format]

EMPIAR-12188

Cryo-EM reconstruction of the full-length H209N mutant E. coli transmembrane formate transporter FocA [2128 multi-frame micrographs composed of 30 frames each in MRC format]

Publication:

Conserved hydrophilic checkpoints tune FocA-mediated formate:H+ symport

Tueting C, Janson K, Michelle K, Ihling C, Kyrilis FL, Hamdi F, Erdmann C, Sinz A, Sawers RG, Kastritis PL

(---)

DOI: 10.1038/s41467-025-65159-3

Related EMDB entry:

EMD-51035

Deposited:

2024-07-15

Released:

2025-10-22

Last modified:

2025-10-22

Imageset size:

991.83 GB

Imageset DOI:

10.6019/EMPIAR-12188

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (FoilHole_*_Fractions.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
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Original size:   2,091,911,248 kilobytes
Compressed size:   964,445,136 kilobytes

Contains:

micrographs - multiframe

Image Sets

1. Unaligned micrographs of the solublized H209N-mutant FocA

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

2128

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.5918, 0.5918)

Details:

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

See all citations

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