EMPIAR-12175 - Cryo-ET dataset of dormant microsporidian spores from Encephalitozoon hellem [multiple data sets in MRCS and MRC formats]

EMPIAR-12175

Cryo-ET dataset of dormant microsporidian spores from Encephalitozoon hellem [multiple data sets in MRCS and MRC formats]

Publication:

Cryo-ET reveals the in situ architecture of the polar tube invasion apparatus from microsporidian parasites

Kelley K, Raczkowski AM, Klykov O, Jaroenlak P, Bobe D, Kopylov M, Eng ET, Bhabha G, Potter CS, Carragher B, Noble AJ, Usmani M, Coudray N, Riggi M, Raghu R, Ramchandani H, Bobe D, Kopylov M, Zhong ED, Iwasa JH, Ekiert DC

Proceedings of the National Academy of Sciences of the United States of America 122 (2025)

PMID: 40067903

DOI: 10.1073/pnas.2415233122

Related EMDB entry:

Deposited:

2024-06-10

Released:

2025-05-22

Last modified:

2025-05-22

Imageset size:

252.12 GB

Imageset DOI:

10.6019/EMPIAR-12175

Experimental metadata:

Download xml json

Contains:

tilt series
reconstructed volumes

Image Sets

1. Aligned tilt series from microsporidian Encephalitozoon hellem

Category:

tilt series

Image format:

MRCS

No. of images or tilt series:

Image size:

(4200, 4200)

Pixel type:

SIGNED 32 BIT INTEGER

Pixel spacing:

(3.298, 3.298)

Details:

Each tilt series in this dataset exists in a unique directory with an identifiable ID. Within each directory is a folder called tilt_series where the corresponding aligned tilt series and tilt file can be found.

Files:

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Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Reconstructed tomograms from microsporidian Encephalitozoon hellem

Category:

reconstructed volumes

Image format:

MRC

No. of images or tilt series:

Image size:

(None, None)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(3.298, 3.298)

Details:

Each reconstructed tomogram in this dataset exists in a unique directory with an identifiable ID. Within each directory is a folder called tomo_rec where the 2X binned reconstruction produced using protomo can be found.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

See all citations

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