EMPIAR-12165 - Cryo-EM structures of AceCas9 [multiple data sets in TIFF format]

EMPIAR-12165

Cryo-EM structures of AceCas9 [multiple data sets in TIFF format]

Publication:

Coupled catalytic states and the role of metal coordination in Cas9

Das A, Rai J, Roth MO, Shu Y, Medina ML, Barakat MR, Li H

Nature catalysis 6 (2023) 969-977

PMID: 38348449

DOI: 10.1038/s41929-023-01031-1

Related EMDB entry:

EMD-27144 EMD-27146 EMD-27142 EMD-27143 EMD-27145 EMD-27141

Deposited:

2024-06-26

Released:

2025-08-13

Last modified:

2025-08-13

Imageset size:

6.35 TB

Imageset DOI:

10.6019/EMPIAR-12165

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unalinged multi-frame micrographs of AceCas9 in super-resolution, dataset 1

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5919

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.412, 0.412)

Details:

Gain file included as k3-gain-20210818.mrc.
No rotation, Flip upsidedown for motion import in RELION; no rotation, no flip in CryoSPARC.

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2. Unalinged multi-frame micrographs of AceCas9 in super-resolution, dataset 2

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7734

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.412, 0.412)

Details:

Gain file saved in dataset 1 folder as k3-gain-20210818.mrc.
No rotation, Flip upsidedown for motion import in RELION; no rotation, no flip in CryoSPARC

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

See all citations

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