EMPIAR-12114 - Scytonema hofmannii TnsC bound to AMPPNP and DNA [multiple data sets in TIFF and DM4 formats]

EMPIAR-12114

Scytonema hofmannii TnsC bound to AMPPNP and DNA [multiple data sets in TIFF and DM4 formats]

Publication:

Target site selection and remodelling by type V CRISPR-transposon systems

Querques I, Schmitz M, Oberli S, Chanez C, Jinek M

Nature 599 (2021) 497-502

PMID: 34759315

DOI: 10.1038/s41586-021-04030-z

Related EMDB entry:

EMD-13489

Deposited:

2024-02-05

Released:

2025-01-28

Last modified:

2025-01-28

Imageset size:

1.43 TB

Imageset DOI:

10.6019/EMPIAR-12114

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame

Image Sets

1. Unaligned multi-frame micrographs of the Scytonema hofmannii TnsC bound to AMPPNP and DNA

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5045

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.325, 0.325)

Details:

The micrographs are the 36 raw movie frames (accumulating 66.39 electrons per squared Angstrom in a 1.0 second exposure).

Files:

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2. Gain reference

Category:

micrographs - single frame

Image format:

DM4

No. of images or tilt series:

Image size:

(11520, 8184)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.325, 0.325)

Details:

Gain reference

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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