EMPIAR-11928 - Cryo electron structure of the ectodomain of SPRING bound to the ectodomain of Site-one protease [4886 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-11928

Cryo electron structure of the ectodomain of SPRING bound to the ectodomain of Site-one protease [4886 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

SPRING licenses S1P-mediated cleavage of SREBP2 by displacing an inhibitory pro-domain

Hendrix S, Dartigue V, Hall H, Bawaria S, Kingma J, Bajaj B, Zelcer N, Kober DL

Nature communications 15 (2024)

PMID: 38977690

DOI: 10.1038/s41467-024-50068-8

Related EMDB entry:

EMD-42639

Deposited:

2023-11-07

Released:

2025-03-17

Last modified:

2025-03-17

Imageset size:

2.93 TB

Imageset DOI:

10.6019/EMPIAR-11928

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. K3 movies for site-one protease complexed to SPRING

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4886

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.08, 1.08)

Details:

Unprocessed movies collected on Titan Krios G2 using bioquantum K3 operating in CDS mode. Superres pixel size is 0.54 A. Collected with a dose rate of 8 eps with a total dose of 48 e/A2. Nominal defocus range of -1 to -2 micron. Data are related to EMD-42639.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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