EMPIAR-11899
Cryo-ET dataset on lamellae of Dictyostelium discoideum cells [213 tilt-series in MRC format] [213 tilt series in MRC format]
Publication:

Thinner is not always better: Optimizing cryo-lamellae for subtomogram averaging

Tuijtel MW, Cruz-León S, Kreysing JP, Welsch S, Hummer G, Beck M, Turoňová B

Science advances 10 (2024)

PMID: 38669330

Related EMDB entry:
Deposited:
2024-02-12
Released:
2024-05-16
Last modified:
2024-05-16
Imageset size:
382.62 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • tilt series
1. Aligned tilt-series of Dictyostelium discoideum cells
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
213
Image size:
(4096, 4096)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(1.223, 1.223)
Details:
Tilt-series were aimed to collect 61 tilts, for some fewer tilts were acquired. Tilt-series are sorted according to ascending tilt angle apart from +60 to -60 from lamella pre-tilt (8deg). mdoc files with metadata provided by SerialEM are also provided.
Tilts that were discarded during processing are specified in "tilt_cleaning.txt".
Alignment results from IMOD are provided as _dose-filt.xf files.
Coordinates to raw ribosome particle picks by template matching are provided in TM_SG_bin6_subtomos_3.1star, with coordinates with bin-factor of 6 corresponding to a pixel size of 7.3 Angstrom. Ribosome particle locations after 3D-classification are provided in Classification_relion_bin6_subtomos_3.1.star, with coordinates with bin-factor of 6 corresponding to a pixel size of 7.3 Angstrom.
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