EMPIAR-11895 - EMinsight: a tool to capture cryoEM microscope configuration and experimental outcomes for analysis and deposition [2605 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-11895

EMinsight: a tool to capture cryoEM microscope configuration and experimental outcomes for analysis and deposition [2605 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

EMinsight: a tool to capture cryoEM microscope configuration and experimental outcomes for analysis and deposition

Morris KL, Harrison PJ, Cha J, Riggs S, Hatton D, Thiyagalingamc J

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Related EMDB entry:

Deposited:

2024-01-05

Released:

2024-04-02

Last modified:

2024-04-02

Imageset size:

1.30 TB

Imageset DOI:

10.6019/EMPIAR-11895

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multiframe micrographs of apoferritin

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2605

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.508, 0.508)

Details:

A SPA data collection structure from eBIC, Diamond Light Source collected on a Titan Krios equipped with Gatan K3. The directory structure contains the unaligned raw movie frames recorded in TIFF format (as output by Thermo Fisher Scientific EPU) in 'data/2023/bi23047-106/raw', EPU metadata in 'data/2023/bi23047-106/atlas' and 'data/2023/bi23047-106/raw/metadata*', on the fly processing from Relion in 'data/2023/bi23047-106/processed' and miscellaneous files in 'data/2023/bi23047-106/processing'.

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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