EMPIAR-11858 - β2-adrenergic receptor in complex with GTP-bound G protein Gs, vitrified 17 seconds after addition of GTP [12930 multi-frame micrographs composed of 79 frames each in TIFF format]

EMPIAR-11858

β2-adrenergic receptor in complex with GTP-bound G protein Gs, vitrified 17 seconds after addition of GTP [12930 multi-frame micrographs composed of 79 frames each in TIFF format]

Publication:

Time-resolved cryo-EM of G-protein activation by a GPCR

Papasergi-Scott MM, Pérez-Hernández G, Batebi H, Gao Y, Eskici G, Seven AB, Panova O, Hilger D, Casiraghi M, He F, Maul L, Gmeiner P, Kobilka BK, Hildebrand PW, Skiniotis G

Nature (2024)

PMID: 38480881

DOI: 10.1038/s41586-024-07153-1

Related EMDB entry:

EMD-40146 EMD-40141 EMD-40143 EMD-40151 EMD-40142 EMD-40153 EMD-40136 EMD-40139 EMD-40140 EMD-29986

Deposited:

2024-01-20

Released:

2024-06-18

Last modified:

2024-06-18

Imageset size:

7.26 TB

Imageset DOI:

10.6019/EMPIAR-11858

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of β2-adrenergic receptor in complex with GTP-bound G protein Gs, vitrified 17 seconds after GTP addition

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

12930

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.43385, 0.43385)

Details:

Raw unaligned movie files (tif format, 79 frames, not gain corrected), and gain correction image (mrc format).
Cryo-EM imaging of the β2AR-Gs(GTP) complex was performed on a Titan Krios electron microscope operated at 300 kV accelerating voltage, and equipped with a K3 Summit direct electron detector and post column energy filter. Super-resolution pixel size: 0.43385Å. Μovies were obtained at an exposure rate of 32.46 electrons/Å^2/sec. The total exposure time was 1.999 sec over 79 frames per movie stack.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Quick Links

Talks and Tutorials

EMPIAR Quick tour

Statistics

Publications

Re-use case study

EMPIAR in the news

Claim entries to your ORCiD

COVID-19 Data Portal

EMDB

PDBe

BioImage Archive

EMNavigator@PDBj

PDBj

RCSB@PDBj

EMDataResoruce@RCSB,EMDB,NCMI

Citations

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

CryoTRANS: predicting high-resolution maps of rare conformations from self-supervised trajectories in cryo-EM.

Fan X, Zhang Q, Zhang H, Zhu J, Ju L, Shi Z, Hu M, Bao C. (2024)

Self-assembled superstructure alleviates air-water interface effect in cryo-EM.

Zheng L, Xu J, Wang W, Gao X, Zhao C, Guo W, Sun L, Cheng H, Meng F, Chen B, Sun W, Jia X, Zhou X, Wu K, Liu Z, Ding F, Liu N, Wang HW, Peng H. (2024)

See all citations

Protein Data Bank Japan News

[2024-09-04]

375 new PDB entries have been released on 2024-09-04.

[2024-08-28]

[wwPDB] Biocurator Milestone: >10,000 Depositions Processed

[2024-08-15]

[wwPDB] PDB Archive Serves Structures Determined by Integrative and Hybrid Methods (IHM)

[2024-06-19]

[wwPDB] Announcing the New PDBx/mmCIF User Guide

[2024-06-11]

[wwPDB] Paper Published on NextGen Archive