EMPIAR-11846 - Single Particle Cryo Electron Microscopy Structure of the Dsl1 Complex bound to the SNAREs Sec20 and Use1 [multiple data sets in MRC format]

EMPIAR-11846

Single Particle Cryo Electron Microscopy Structure of the Dsl1 Complex bound to the SNAREs Sec20 and Use1 [multiple data sets in MRC format]

Publication:

Structure of a Membrane Tethering Complex Incorporating Multiple SNAREs

DAmico KA, Stanton AE, Shirkey JD, Travis SM, Jeffrey PD, Hughson FM

bioRxiv (2023)

PMID: 36778436

DOI: 10.1101/2023.01.30.526244

Related EMDB entry:

EMD-28204

Deposited:

2023-12-13

Released:

2024-03-16

Last modified:

2024-03-16

Imageset size:

1.39 TB

Imageset DOI:

10.6019/EMPIAR-11846

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies (Movies/*/FoilHole*.mrc) in this entry were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

Compression was carried out by separating (“un-normalizing”) the gain.
The details of the method are described in
https://relion.readthedocs.io/en/release-3.1/Reference/MovieCompression.html#gain-normalised-movies.

Please apply the gain reference (gain-reference.mrc in each subdirectory) during processing.

Original size:   10,087,878,962 kilobytes
Compressed size:  1,202,493,003 kilobytes

Contains:

micrographs - multiframe
picked particles - single frame - processed

Image Sets

1. Raw micrographs of the Dsl1:Qb:Qc complex

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

5857

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.114, 1.114)

Details:

unprocessed micrographs with accumulating 45 electron per squared Angstrom

Files:

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2. Extracted particles of the Dsl1:Qb:Qc complex

Category:

picked particles - single frame - processed

Image format:

MRC

No. of images or tilt series:

469193

Image size:

(400, 400)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.114, 1.114)

Details:

Extracted particles with box size 400 pixels

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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