EMPIAR-11819 - Cryo-electron tomogram of mitochondria in cryo-FIB milled l-Opa1* mouse embryonic fibroblasts [245 multi-frame micrographs composed of 6 frames each in TIFF format]

EMPIAR-11819

Cryo-electron tomogram of mitochondria in cryo-FIB milled l-Opa1* mouse embryonic fibroblasts [245 multi-frame micrographs composed of 6 frames each in TIFF format]

Publication:

In situ architecture of Opa1-dependent mitochondrial cristae remodeling

Fry MY, Navarro PP, Hakim P, Ananda VY, Qin X, Landoni JC, Rath S, Inde Z, Lugo CM, Luce BE, Ge Y, McDonald JL, Ali I, Ha LL, Kleinstiver BP, Chan DC, Sarosiek KA, Chao LH

The EMBO journal 43 (2024) 391-413

PMID: 38225406

DOI: 10.1038/s44318-024-00027-2

Related EMDB entry:

EMD-43052 EMD-43049 EMD-43051 EMD-43050

Deposited:

2023-12-07

Released:

2024-06-05

Last modified:

2024-06-05

Imageset size:

20.16 GB

Imageset DOI:

10.6019/EMPIAR-11819

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame movies of tilt-series of cryo-FIB milled Mouse Embryonic Fibroblasts with OPA1 exon5b deletion and OMA1 deletion

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

245

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.375, 1.375)

Details:

Unaligned multi-frame micrographs (EMD-430*/frames/*.tiff) corresponding to 4 tilt series with corresponding gain reference (gain/*dm4) and metadata (mdoc/*.mdoc). Data acquired on a K3 BioQuantum detector with no Volta phase plate.

Acquisition parameters differ for each dataset (named after the corresponding EMDB accession code) and are specified below.

EMD-43049:
62 tilts in the series.

EMD-43050:
61 tilts in the series

EMD-43051:
61 tilts in the series

EMD-43052:
61 tilts in the series

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

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