EMPIAR-11777
CryoEM Structure of Bombus Flight Muscle Thick Filaments at 6Å Resolution [4476 micrographs in MRC format]
Publication:

Structure of the Flight Muscle Thick Filament from the Bumble Bee, Bombus ignitus, at 6 Å Resolution

Li J, Rahmani H, Abbasi Yeganeh F, Rastegarpouyani H, Taylor DW, Wood NB, Previs MJ, Iwamoto H, Taylor KA

International journal of molecular sciences 24 (2022) 377-25

PMID: 36613818

Related EMDB entry:
Deposited:
2023-10-11
Released:
2025-07-16
Last modified:
2025-07-16
Imageset size:
4.10 TB
Imageset DOI:
Experimental metadata:
Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (data/20200321_*.mrc*) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

Original size:   15,453,962,928 kilobytes
Compressed size:  8,362,384,457 kilobytes

NOTE: The original deposition had several issues. PDBj treated them as follows.

- The `Stack` folder contained temporary files starting with ".".
  PDBj deleted the folder.

- The `Stacks` folder contained 214 files that were duplicates of the files in the parent directory.
  PDBj deleted the folder.

- The top directory (`data`) contained many .mrc and .mrcs files.
  Most of them were integer movies but the following eight files were floating-point single-frame
  images. PDBj compressed the movies but left the eight signle-frame images uncompressed.
  - data/20200321_1312_A016_G000_H000_D001.mrc
  - data/20200321_1312_A016_G000_H000_D002.mrc
  - data/20200321_1312_A016_G000_H000_D003.mrc
  - data/20200321_1312_A016_G000_H000_D004.mrc
  - data/20200321_1312_A016_G000_H002_D001.mrc
  - data/20200321_1312_A016_G000_H002_D002.mrc
  - data/20200321_1312_A016_G000_H002_D003.mrc
  - data/20200321_1312_A016_G000_H002_D004.mrc
Contains:
  • micrographs - single frame
1. Single particle cryo-EM dataset of flight muscle thick filaments from Bomble Bee, Bombus ignitus [multiple data sets in MRC, MRCS]
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
4476
Image size:
(None, None)
Pixel type:
SIGNED 32 BIT INTEGER
Pixel spacing:
(None, None)
Details:
---
Files:
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Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)
Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)
Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)
Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)