EMPIAR-11762 - Cryo-EM structure of human TFIIIC bound and unbound to tRNA gene [multiple data sets in TIFF and MRCS formats]

EMPIAR-11762

Cryo-EM structure of human TFIIIC bound and unbound to tRNA gene [multiple data sets in TIFF and MRCS formats]

Publication:

Structural insights into human TFIIIC promoter recognition

Seifert-Davila W, Girbig M, Hauptmann L, Hoffmann T, Eustermann S, Müller CW

Science advances 9 (2023)

PMID: 37418517

DOI: 10.1126/sciadv.adh2019

Related EMDB entry:

EMD-16713 EMD-16717 EMD-16715 EMD-17447 EMD-16716 EMD-17446 EMD-16714

Deposited:

2023-11-02

Released:

2024-02-28

Last modified:

2024-02-28

Imageset size:

3.77 TB

Imageset DOI:

10.6019/EMPIAR-11762

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
picked particles - single frame - processed

Image Sets

1. Unaligned multi-frame micrographs of human TFIIIC bound to tRNA gene

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

11025

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.822, 0.822)

Details:

Unaligned multi-frame (40 frames) micrographs were collected in counting mode with a total electron dose of 42.8 electrons/Å2 at defocus range from 0.7 to 1.7 . Gain reference is in CountRef_211108_hTFIIIC_DNA_00001.mrc file.

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2. Final particles of TauA subcomplex

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

55079

Image size:

(352, 352)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.822, 0.822)

Details:

The directory "data/Dataset_1/TauA_final_particles" contains the ".mrcs" files of the final 3D reconstruction of TauA. These particles were also used for the "Cryo-EM mapping of particle pair distances at a single-molecule level" with TauB-dimer particles. The file "particles.star" contains the coordinates.

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3. Final particles of TauB-dimer subcomplex

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

35379

Image size:

(480, 480)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.822, 0.822)

Details:

The directory "data/Dataset_1/TauB_dimer_final_particles" contains the ".mrcs" files of the final 3D reconstruction of TauB-dimer. The file "particles.star" contains the coordinates.

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4. Intermediate particles of TauB-dimer subcomplex for mapping pair distance

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

99217

Image size:

(480, 480)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.822, 0.822)

Details:

The directory "data/Dataset_1/TauB_dimer_pair_distances" contains the ".mrcs" files of Non-Uniform refinement reconstruction of TauB-dimer. These particles were used for the "Cryo-EM mapping of particle pair distances at a single-molecule level" with TauA particles. The file "particles.star" contains the coordinates.

Files:

This entry cannot be downloaded from PDBj.

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5. Unaligned multi-frame micrographs of human TFIIIC (No DNA sample)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

11088

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.822, 0.822)

Details:

Unaligned multi-frame (40 frames) micrographs were collected in counting mode with a total electron dose of 39.6 electrons/Å2 at defocus range from 0.7 to 1.7 . Gain reference is in CountRef_hTFIIIC_OG_113-1_00001.mrc file.

Files:

This entry cannot be downloaded from PDBj.

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6. Final particles of TauA subcomplex (No DNA sample)

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

94103

Image size:

(300, 300)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.822, 0.822)

Details:

The directory "data/Dataset_2/TauA_noDNA_final_particles" contains the ".mrcs" files of the final 3D reconstruction of TauA. The file "particles.star" contains the coordinates.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

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7. Final particles of TauB-dimer subcomplex (No DNA sample)

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

125600

Image size:

(480, 480)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.822, 0.822)

Details:

The directory "data/Dataset_2/TauB_dimer_noDNA_final_particles" contains the ".mrcs" files of the final 3D reconstruction of TauA. The file "particles.star" contains the coordinates.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

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