EMPIAR-11742 - Cryo-EM SPA dataset of Streptococcus pneumoniae NADPH oxidase with NADPH under turnover conditions [22290 multi-frame micrographs composed of 1036 frames each in EER format]

EMPIAR-11742

Cryo-EM SPA dataset of Streptococcus pneumoniae NADPH oxidase with NADPH under turnover conditions [22290 multi-frame micrographs composed of 1036 frames each in EER format]

Publication:

Structural and mechanistic insights into Streptococcus pneumoniae NADPH oxidase

Dubach VRA, San Segundo-Acosta P, Murphy BJ

Nature structural & molecular biology (2024)

PMID: 39039317

DOI: 10.1038/s41594-024-01348-w

Related EMDB entry:

EMD-18645

Deposited:

2023-10-16

Released:

2025-07-10

Last modified:

2025-07-10

Imageset size:

9.36 TB

Imageset DOI:

10.6019/EMPIAR-11742

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned mutliframe non-gain-normalized EER movies of SpNOX with NADPH collected on a Krios G4

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

22290

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.573, 0.573)

Details:

Raw EER movies collected using a Krios G4 with a CFEG, Falcon 4 detector and Selectris X filter.

The EER movies contain 1036 frames and the total exposure dose of the sample was 70 e/A2. Magnification used was 215kx with a pixel size of 0.573 Å. Energy slit was set to 10 eV.

XML files to generate optics groups are provided as well as the gain reference.

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Citations

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Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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