EMPIAR-11722 - Cyo-EM structure of wildtype non-gastric proton pump in the presence of Na+, AlF and ADP [multiple data sets in TIFF format]

EMPIAR-11722

Cyo-EM structure of wildtype non-gastric proton pump in the presence of Na+, AlF and ADP [multiple data sets in TIFF format]

Publication:

An unusual conformation from Na + -sensitive non-gastric proton pump mutants reveals molecular mechanisms of cooperative Na + -binding.

Abe K, Nishizawa T, Artigas P

Biochimica Biophysica Acta Molecular Cell Research 1870 (2023)

PMID: 37482134

DOI: 10.1016/j.bbamcr.2023.119543

Related EMDB entry:

EMD-35488 EMD-35489 EMD-35491

Deposited:

2023-10-11

Released:

2024-08-23

Last modified:

2024-08-23

Imageset size:

1.71 TB

Imageset DOI:

10.6019/EMPIAR-11722

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. K3 movies for non gastric proton pump WT with Na, AlF and ADP

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1723

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

- Please use 'movie_gain_ref_estimate.mrc' as a gain reference without rotation or flipping in RELION.
- The gain reference (movie_gain_ref_estimate.mrc) was generated with relion_estimate_gain by averaging many frames.
- The flux is 1.06 e/A2/frame.
- Cs is 2.7 mm.
- Four digits in the file name represent image shift directions.
- Movies were recorded with 3x3 image shift but grouping was not applied during processing.

Files:

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2. K3 movies for non gastric proton pump K794A mutant in NaE1AMPPCP state

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3204

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

- Please use 'CountRef_abe1-3_11-001_0000_Sep09_09.26.25.mrc' as a gain reference without rotation or flipping in RELION.
- The flux is 1.06 e/A2/frame.
- Cs is 2.7 mm.
- Four digits in the file name represent image shift directions.
- Movies were recorded with 3x3 image shift but grouping was not applied during processing.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. K3 movies for non gastric proton pump K794S mutant in NaE1AMPPCP state

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2187

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

- Please use 'CountRef_abe2-1_22-001_0000_Sep10_01.28.37.mrc' as a gain reference without rotation or flipping in RELION.
- The flux is 1.06 e/A2/frame.
- Cs is 2.7 mm.
- Four digits in the file name represent image shift directions.
- Movies were recorded with 3x3 image shift but grouping was not applied during processing.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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