EMPIAR-11715 - CryoEM movies of IAPP-S20G timecourse (6 week, FT11) [2350 multi-frame micrographs composed of 30 frames each in TIFF format]

EMPIAR-11715

CryoEM movies of IAPP-S20G timecourse (6 week, FT11) [2350 multi-frame micrographs composed of 30 frames each in TIFF format]

Publication:

Structural evolution of fibril polymorphs during amyloid assembly

Wilkinson M, Xu Y, Thacker D, Taylor AIP, Fisher DG, Gallardo RU, Radford SE, Ranson NA

Cell 186 (2023) 5798-5811.e26

PMID: 38134875

DOI: 10.1016/j.cell.2023.11.025

Related EMDB entry:

EMD-15730 EMD-15729 EMD-15731 EMD-15728

Deposited:

2023-08-03

Released:

2024-04-17

Last modified:

2024-04-17

Imageset size:

316.15 GB

Imageset DOI:

10.6019/EMPIAR-11715

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned tif movie stacks (gain corrected) for the IAPP-S20G 6 week cryoEM dataset

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2350

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.84, 0.84)

Details:

Only unaligned movies are present. The total dose was 43 e-/Å2 over 30 frames. At least six fibril polymorphs were identified, with four structures resolved (2PFc, 2PFl, 3PFcu & 4PFcu).

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

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