EMPIAR-11714 - CryoEM movies of IAPP-S20G timecourse (3 week, FT24) [495 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-11714

CryoEM movies of IAPP-S20G timecourse (3 week, FT24) [495 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Structural evolution of fibril polymorphs during amyloid assembly

Wilkinson M, Xu Y, Thacker D, Taylor AIP, Fisher DG, Gallardo RU, Radford SE, Ranson NA

Cell 186 (2023) 5798-5811.e26

PMID: 38134875

DOI: 10.1016/j.cell.2023.11.025

Related EMDB entry:

EMD-15696

Deposited:

2023-08-03

Released:

2024-04-17

Last modified:

2024-04-17

Imageset size:

75.24 GB

Imageset DOI:

10.6019/EMPIAR-11714

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned tif movie stacks (gain corrected) for the IAPP-S20G 3 week cryoEM dataset

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

495

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.9, 0.9)

Details:

Only unaligned movies are present. The total dose was 39 e-/Å2 over 40 frames. A single polymorph was resolved (2PFp, ~30% of the fibrils).

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Citations

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Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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