EMPIAR-11707 - Substrate-free AAA single-chained ClpX∆N protein in a complex with ClpP peptidase [multiple data sets in TIFF and MRC formats]

EMPIAR-11707

Substrate-free AAA single-chained ClpX∆N protein in a complex with ClpP peptidase [multiple data sets in TIFF and MRC formats]

Publication:

A closed translocation channel in the substrate-free AAA+ ClpXP protease diminishes rogue degradation

Ghanbarpour A, Cohen SE, Fei X, Sauer RT, Davis JH

Nature Communications (2023)

DOI: 10.1101/2022.08.27.505532

Related EMDB entry:

EMD-27946

Deposited:

2023-08-13

Released:

2024-08-22

Last modified:

2024-08-22

Imageset size:

894.90 GB

Imageset DOI:

10.6019/EMPIAR-11707

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
picked particles - single frame - processed

Image Sets

1. Unaligned Multiframes Micrographs

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2405

Image size:

(8184, 11520)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.416, 0.416)

Details:

Defocus range (min, max, step):-0.75, -2.5, -0.25
Total exposure on specimen: 49.39 el/A2
Objective lens spherical aberration (Cs): 2.7mm
Acceleration voltage: 300kV
uncalibrated pixel size: 0.435 Å
calibrated pixel size: 0.416 Å

Files:

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2. Particle stacks of substrate-free ClpX(deltaN domain).ClpP (singly capped)

Category:

picked particles - single frame - processed

Image format:

MRC

No. of images or tilt series:

49824

Image size:

(300, 300)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.305, 1.305)

Details:

Final Particle stack for EMD-27941 (singly capped ClpX-deltaN.ClpP)

Files:

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3. Particle stacks of substrate-free ClpX(delta-N domain).ClpP (doubly capped)

Category:

picked particles - single frame - processed

Image format:

MRC

No. of images or tilt series:

171869

Image size:

(256, 256)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.106, 1.106)

Details:

Final Particle stack for EMD-27946 (doubly capped ClpX-deltaN.ClpP)

Files:

This entry cannot be downloaded from PDBj.

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Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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