EMPIAR-11647
Cryo-EM structure of human DNA polymerase alpha-primase in pre-initiation stage 2 to 4 and initiation [7014 multi-frame micrographs composed of 45 frames each in TIFF format]
Publication:

CryoEM insights into RNA primer synthesis by the human primosome

Yin Z, Kilkenny ML, Ker DS, Pellegrini L

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Related EMDB entry:
Deposited:
2023-08-02
Released:
2024-07-03
Last modified:
2024-07-03
Imageset size:
1.70 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of human DNA polymerase alpha-primase
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
7014
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.066, 1.066)
Details:
Micrographs were imaged using Gatan K3 camera, each with 45 movie frames using the FEI EPU software with aberration-free image shift. The calibrated pixel size was 1.066 Å, and the total electron dose was 50.50 electrons Å-2 for each micrograph.
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