EMPIAR-11637 - Single particle cryo-EM dataset of Bos taurus mitochondrial complex I (originally purified in LMNG) proteoliposomes and deactivated [6692 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-11637

Single particle cryo-EM dataset of Bos taurus mitochondrial complex I (originally purified in LMNG) proteoliposomes and deactivated [6692 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Molecular mechanism of the ischemia-induced regulatory switch in mammalian complex I

Grba DN, Wright JJ, Fisher W, Yin Z, Hirst J

Science (New York, N.Y.) 384 (2024) 1247-1253

PMID: 38870289

DOI: 10.1126/science.ado2075

Related EMDB entry:

EMD-18068 EMD-18069 EMD-18066 EMD-18067

Deposited:

2023-08-01

Released:

2024-07-09

Last modified:

2024-07-09

Imageset size:

1.38 TB

Imageset DOI:

10.6019/EMPIAR-11637

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of wild-type bos taurus mitochondrial complex I, solubilised in LMNG, reconstituted into proteoliposomes and deactivated. Non-tilted collection.

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6692

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.072, 1.072)

Details:

Unaligned multi-frame micrographs of wild-type bovine mitochondrial complex I from an as-prepared sample solubilised in LMNG and reconstituted in to liposomes. Not gain corrected (gain file included). The proteoliposomes were heat treated (37 ºC, 20 min) to deactivate the sample, before being frozen on graphene oxide gold grids. Images (TIFF) were collected with a 300 keV FEI Titan Krios on a Gatan K3 detector in super-resolution mode but bin2 at the hardware stage, using counting mode and a Gatan BioContinuum energy filter (20 eV). The C2 and objective lens apertures were set to 50 and 100 µm, respectively. 3.8 s exposures were captured in 40 frames with a total dose of ~40 Å-2. A pixel size of 1.072 Å/pixel was used.

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Citations

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