EMPIAR-11581 - Cryo electron microscopy micrographs of high molecular weight fractions from yeast native cell extracts [51497 multi-frame micrographs composed of 972 frames each in EER format]

EMPIAR-11581

Cryo electron microscopy micrographs of high molecular weight fractions from yeast native cell extracts [51497 multi-frame micrographs composed of 972 frames each in EER format]

Publication:

Delineating organizational principles of the endogenous L-A virus by cryo-EM and computational analysis of native cell extracts

Schmidt L, Tüting C, Kyrilis FL, Hamdi F, Semchonok DA, Hause G, Meister A, Ihling C, Stubbs MT, Sinz A, Kastritis PL

Communications biology 7 (2024)

PMID: 38730276

DOI: 10.1038/s42003-024-06204-7

Related EMDB entry:

EMD-17628

Deposited:

2022-12-13

Released:

2024-05-29

Last modified:

2024-06-17

Imageset size:

23.85 TB

Imageset DOI:

10.6019/EMPIAR-11581

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. unaligned multi-frame micrographs of a high-molecular yeast fraction

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

51497

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.93, 0.93)

Details:

This dataset provides the cryo-electron microscopy (cryo-EM) analysis of high molecular weight biomolecules isolated from Saccharomyces cerevisiae, commonly known as baker's yeast. The cells were grown, lysed, and the lysate was subsequently cleared and fractionated using size exclusion chromatography. The data focuses on fraction #5, known for its rich composition of ribosomes and L-A virus capsids. The cryo-EM data presented here offers a comprehensive visualization of these essential biomolecular structures, providing an insightful perspective into their structural organization and potentially informing future studies of yeast cellular processes and viral interactions.

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This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

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Citations

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

CryoTRANS: predicting high-resolution maps of rare conformations from self-supervised trajectories in cryo-EM.

Fan X, Zhang Q, Zhang H, Zhu J, Ju L, Shi Z, Hu M, Bao C. (2024)

Self-assembled superstructure alleviates air-water interface effect in cryo-EM.

Zheng L, Xu J, Wang W, Gao X, Zhao C, Guo W, Sun L, Cheng H, Meng F, Chen B, Sun W, Jia X, Zhou X, Wu K, Liu Z, Ding F, Liu N, Wang HW, Peng H. (2024)

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