EMPIAR-11547 - Cryo electron microscopy of Virus-like Particle based on PVY coat protein with dC79 deletion [491 multi-frame micrographs composed of 41 frames each in TIFF format]

EMPIAR-11547

Cryo electron microscopy of Virus-like Particle based on PVY coat protein with dC79 deletion [491 multi-frame micrographs composed of 41 frames each in TIFF format]

Publication:

From structural polymorphism to structural metamorphosis of the coat protein of flexuous filamentous potato virus Y

Kavčič L, Kežar A, Koritnik N, Žnidarič MT, Klobučar T, Vičič Ž, Merzel F, Holden E, Benesch JLP, Podobnik M

Communications chemistry 7 (2024)

PMID: 38233506

DOI: 10.1038/s42004-024-01100-x

Related EMDB entry:

EMD-17052

Deposited:

2023-04-25

Released:

2024-02-13

Last modified:

2024-02-13

Imageset size:

377.40 GB

Imageset DOI:

10.6019/EMPIAR-11547

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of Virus-like Particles based on PVY coat protein with dC79 deletion

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

491

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.95, 0.95)

Details:

The movies were imaged (Titan Glacios) at a nominal pixel resolution of 0.976 Å/pixel using a Falcon 3 direct electron detector (Thermo Fisher Scientific) in counting mode. Each movie is collected across 40 frames with a total electron dose of 42 e-/Å2. After pixel size calibration, the calibrated pixel size is 0.950 A.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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