EMPIAR-11510 - Cryo-EM structure of human pseudouridine synthase 3 [8211 micrographs in TIFF format]

EMPIAR-11510

Cryo-EM structure of human pseudouridine synthase 3 [8211 micrographs in TIFF format]

Publication:

The molecular basis of tRNA selectivity by human pseudouridine synthase 3

Lin TY, Kleemann L, Jeżowski J, Dobosz D, Rawski M, Indyka P, Ważny G, Mehta R, Chramiec-Głąbik A, Koziej Ł, Ranff T, Fufezan C, Wawro M, Kochan J, Bereta J, Leidel SA, Glatt S

Molecular cell 84 (2024) 2472-2489.e8

PMID: 38996458

DOI: 10.1016/j.molcel.2024.06.013

Related EMDB entry:

EMD-16917

Deposited:

2023-03-28

Released:

2024-08-26

Last modified:

2024-08-26

Imageset size:

3.77 TB

Imageset DOI:

10.6019/EMPIAR-11510

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Human PUS3 apo structure

Category:

micrographs - single frame

Image format:

TIFF

No. of images or tilt series:

8211

Image size:

(None, None)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(None, None)

Details:

high tension 300kV
spherical aberration 2.7mm
pixel size 0.86 A/px
total dose on vacuum 40.06 e/A2
frames per movie 40

defocus range set -1.5, -1.2, -0.9 um

sample tilt 0 deg

imaging mode EFTEM
magnification of hole image 11500x
magnification of exposure image 105000x
beam size 0.75 um
C2 aperture 50um
objective aperture retracted

Gatan BioQuantum energy filter
energy slit 20eV
Gatan K3 counting mode
dose rate on vacuum 16.35 e/px/s
exposure image resolution 5760 x 4092
physical pixel (Super Resolution off)
image type .tiff (gain corrected)

EPU version 2.10.0.1941REL

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

CryoPROS: Correcting misalignment caused by preferred orientation using AI-generated auxiliary particles.

Zhang H, Zheng D, Wu Q, Yan N, Peng H, Hu Q, Peng Y, Yan Z, Shi Z, Bao C, Hu M. (2025)

Few-shot learning for non-vitrified ice segmentation.

Vivas-Lago A, Castaño-Díez D. (2025)

Automated removal of corrupted tilts in cryo-electron tomography.

Majtner T, Turoňová B. (2025)

Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with both 2:1 and 2:2 stoichiometries.

Felix J, De Munck S, Bazan JF, Savvides SN. (2025)

Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE.

Van CTS, Reboul CF, Caesar JJE, Meana-Pañeda R, Lountos GT, Deme JC, Bryant OJ, Johnson S, Piczak CT, Valkov E, Lea SM, Elmlund H. (2025)

Cryo-EM Structure Reconstruction by Gaussian Splatting: Pushing the Resolution to Extreme

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2025)

Molecular exaptation by the integrin αI domain.

Hollis JA, Chan MC, Malik HS, Campbell MG. (2025)

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