EMPIAR-11439 - Cryo-Electron Microscopy structure of OmcZ nanowires from Geobacter sulfurreducens [14005 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-11439

Cryo-Electron Microscopy structure of OmcZ nanowires from Geobacter sulfurreducens [14005 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

Structure of Geobacter cytochrome OmcZ identifies mechanism of nanowire assembly and conductivity

Gu Y, Guberman-Pfeffer MJ, Srikanth V, Shen C, Giska F, Gupta K, Londer Y, Samatey FA, Batista VS, Malvankar NS

Nature microbiology 8 (2023) 284-298

PMID: 36732469

DOI: 10.1038/s41564-022-01315-5

Related EMDB entry:

EMD-23481

Deposited:

2022-10-06

Released:

2023-04-12

Last modified:

2023-04-13

Imageset size:

6.46 TB

Imageset DOI:

10.6019/EMPIAR-11439

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs using super resolution mode of OmcZ nanowire

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

14005

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.411, 0.411)

Details:

Particles are autopicked helical particles generated by RELION 3.0.8. Micrographs are raw unaligned movies in super-resolution mode with a physical pixel size of 0.828 Å. Each micrograph contains 50 frames with a total dose of 60 e-/Å2. Super.mrc can be used as the gain reference for downstream motion correction.

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The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

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