EMPIAR-11406 - Hexameric human IgG3 Fc complex [stack of 1193 particles in MRC format]

EMPIAR-11406

Hexameric human IgG3 Fc complex [stack of 1193 particles in MRC format]

Publication:

Complement is activated by elevated IgG3 hexameric platforms and deposits C4b onto distinct antibody domains

Abendstein LA

(---)

Related EMDB entry:

EMD-16227

Deposited:

2023-01-30

Released:

2023-06-23

Last modified:

2023-06-23

Imageset size:

25.92 GB

Imageset DOI:

10.6019/EMPIAR-11406

Experimental metadata:

Download xml json

Contains:

picked particles - multiframe - processed

Image Sets

1. extracted particles - IgG3 bound to DNP representing liposomes

Category:

picked particles - multiframe - processed

Image format:

MRC

No. of images or tilt series:

1193

Image size:

(180, 180)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(3.48, 3.48)

Details:

Tilt series were collected on a Talos Arctica (200kV) microscope with a K3 direct electron detector and a Bioquantum energy filter (slit width=20 eV; Gatan). Tomograms were reconstructed using weighed back-projection in IMOD4.11.Particles were manually picked in EMAN2 (version 2.91) using 2spt_boxer_old.py. 1,193 particles were extracted from 55 tomograms with a box size of 180 pixels and a final pixel size of 3.48 Å/pixel.

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

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