EMPIAR-11405 - Cryo-EM structure of the ribosome/Sec61 translocon with a macrocyclic inhibitor KZR-8445 [multiple data sets in TIFF and MRC formats]

EMPIAR-11405

Cryo-EM structure of the ribosome/Sec61 translocon with a macrocyclic inhibitor KZR-8445 [multiple data sets in TIFF and MRC formats]

Publication:

Signal peptide mimicry primes Sec61 for client-selective inhibition

Rehan S, Tranter D, Sharp PP, Lowe E, Anderl JL, Muchamuel T, Abrishami V, Kuivanen S, Wenzell N, Jennings A, Kalyanaraman C, Craven GB, Strandin T, Javanainen M, Vapalahti O, Jacobson M, McMinn D, Kirk CJ, Huiskonen JT, Taunton J, Paavilainen VO

bioRxiv (2022)

DOI: 10.1101/2022.07.03.498529

Related EMDB entry:

EMD-14776

Deposited:

2023-01-13

Released:

2023-03-27

Last modified:

2023-03-28

Imageset size:

12.86 TB

Imageset DOI:

10.6019/EMPIAR-11405

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame

Image Sets

1. Unaligned micrographs of the ribosome/Sec61/KZR-8445 complex

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

30260

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.415, 0.415)

Details:

The micrographs are the average, without any realignment of 50 movie frames (accumulating 47.66 electrons per square Ångstrom). Data was collected in super resolution mode (physical pixel size 0.83 px/Å) and binned 2x during preprocessing. Data collected on a Krios instrument with a K3 detector operated at 300 kV and at a nominal magnification of 105000x.

Details:
Nominal Defocus range (min, max, step): -0.6, -2.2
Total exposure on specimen: 47.66 e/A^2
Exposure time: 3.0 s
Nominal pixel size: 0.83 A (0.415 A super res)
Objective lens spherical aberration (Cs): 2.7 mm
Acceleration voltage: 300 kV
Microscope / Detector: 300 kV FEI Titan Krios TEM / Gatan K3 summit

Files:

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2. Motion corrected micrographs of the ribosome/Sec61/KZR-8445 complex

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

30260

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

Positions of particles for the final reconstructed volume in associated .star file.

Files:

This entry cannot be downloaded from PDBj.

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We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

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Citations

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Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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