EMPIAR-11377 - Translating 70S ribosome [19449 multi-frame micrographs composed of 1295 frames each in EER format]

EMPIAR-11377

Translating 70S ribosome [19449 multi-frame micrographs composed of 1295 frames each in EER format]

Publication:

The translating bacterial ribosome at 1.55 Å resolution by open access cryo-EM

Fromm SA, O'Connor KM, Purdy M, Bhatt PR, Loughran G, Atkins JF, Jomaa A, Mattei S

BioRxiv (---)

DOI: 10.1101/2022.08.30.505838

Related EMDB entry:

EMD-15793

Deposited:

2022-09-09

Released:

2023-01-30

Last modified:

2023-01-30

Imageset size:

7.11 TB

Imageset DOI:

10.6019/EMPIAR-11377

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Translating 70S ribosome

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

19449

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.731, 0.731)

Details:

total dose is 40 e/A^2; 16 exposures per stage shift using beam image shift in SerialEM; movie suffix *_1-1.eer to *_1-16.eer reflects the respective position in the multi-shot exposure pattern. Movies are not gain corrected. Gain reference is provided: 20211220_115912_EER_GainReference.gain

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

See all citations

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