EMPIAR-11370 - A surface morphometrics toolkit to quantify organellar membrane ultrastructure using cryo-electron tomography [multiple data sets in MRC format]

EMPIAR-11370

A surface morphometrics toolkit to quantify organellar membrane ultrastructure using cryo-electron tomography [multiple data sets in MRC format]

Publication:

A surface morphometrics toolkit to quantify organellar membrane ultrastructure using cryo-electron tomography

Barad BA, Medina M, Fuentes D, Wiseman RL, Grotjahn DA

bioRxiv (2022)

DOI: 10.1101/2022.01.23.477440

Related EMDB entry:

Deposited:

2023-01-06

Released:

2023-02-10

Last modified:

2023-02-10

Imageset size:

126.86 GB

Imageset DOI:

10.6019/EMPIAR-11370

Experimental metadata:

Download xml json

Contains:

tilt series
reconstructed volumes

Image Sets

1. Unaligned tilt series of mouse embrionic fibroblasts with mitochondrial targeted GFP treated with vehicle or thapsigargin

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(None, None)

Details:

Single tilt axis tilt series. Tilt range is variable. For tilt series where frames were acquired the number of frames is 10 and the motioncorrected tilt series is given. Tilt series are from multiple data sets and have either a pixel spacing of 3.345 or 3.11, please refer to associated .mdoc file.

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2. Reconstructed tomograms used for segmentation of mitochondria and endoplasmic reticulum membranes

Category:

reconstructed volumes

Image format:

MRC

No. of images or tilt series:

Image size:

(960, 928)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(None, None)

Details:

Bin 4 reconstructed volumes from tilt series.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

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