EMPIAR-11370
A surface morphometrics toolkit to quantify organellar membrane ultrastructure using cryo-electron tomography [multiple data sets in MRC format]
Publication:

A surface morphometrics toolkit to quantify organellar membrane ultrastructure using cryo-electron tomography

Barad BA, Medina M, Fuentes D, Wiseman RL, Grotjahn DA

bioRxiv (2022)

Related EMDB entry:
Deposited:
2023-01-06
Released:
2023-02-10
Last modified:
2023-02-10
Imageset size:
136.22 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • tilt series
  • reconstructed volumes
1. Unaligned tilt series of mouse embrionic fibroblasts with mitochondrial targeted GFP treated with vehicle or thapsigargin
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
34
Image size:
(3710, 3838)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Single tilt axis tilt series. Tilt range is variable. For tilt series where frames were acquired the number of frames is 10 and the motioncorrected tilt series is given. Tilt series are from multiple data sets and have either a pixel spacing of 3.345 or 3.11, please refer to associated .mdoc file.
Files:
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2. Reconstructed tomograms used for segmentation of mitochondria and endoplasmic reticulum membranes
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
34
Image size:
(960, 928)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Bin 4 reconstructed volumes from tilt series.
Files:
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