EMPIAR-11351 - Cryo-EM structures of the β3 adrenergic receptor bound to solabegron and isoproterenol [multiple data sets in TIFF format]

EMPIAR-11351

Cryo-EM structures of the β3 adrenergic receptor bound to solabegron and isoproterenol [multiple data sets in TIFF format]

Publication:

Cryo-EM structures of the β3 adrenergic receptor bound to solabegron and isoproterenol

Nureki I, Kobayashi K, Tanaka T, Demura K, Inoue A, Shihoya W, Nureki O

Biochemical and biophysical research communications 611 (2022) 158-164

PMID: 35489202

DOI: 10.1016/j.bbrc.2022.04.065

Related EMDB entry:

EMD-33227 EMD-33228

Deposited:

2022-12-07

Released:

2023-01-16

Last modified:

2023-01-16

Imageset size:

2.52 TB

Imageset DOI:

10.6019/EMPIAR-11351

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. Unaligned, non-dose weighted raw movies for B3AR with isoproterenol (EMD-33227)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3789

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

- 300 kV
- Cs 2.7 mm
- 50 e/Å2 over 64 frames

Please use "20210409_Nureki_ikko2_CDS_data/CountCDSRef_ikko2_82-1_0000_Apr09_14.47.39.mrc" as a gain reference without rotation or flipping.
Please exclude the first frame in each movie, which was corrupted due to a bug in the CDS mode.

Four digits (0000 to 0008) in the file names represent the direction of beam shift.

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Unaligned, non-dose weighted raw movies for B3AR with solabegron (EMD-33228)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4833

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

- 300 kV
- Cs 2.7 mm
- 50 e/Å2 over 64 frames

Please use "20210113_Nureki_Ikko1-3_data/CountCDSRef_ikko1-3_47-1_0000_Jan13_18.08.04.mrc" as a gain reference without rotation or flipping.
Please exclude the first frame in each movie, which was corrupted due to a bug in the CDS mode.

Four digits (0000 to 0008) in the file names represent the direction of beam shift.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Quick Links

Talks and Tutorials

EMPIAR Quick tour

Statistics

Publications

Re-use case study

EMPIAR in the news

Claim entries to your ORCiD

COVID-19 Data Portal

EMDB

PDBe

BioImage Archive

EMNavigator@PDBj

PDBj

RCSB@PDBj

EMDataResoruce@RCSB,EMDB,NCMI

Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

See all citations

Protein Data Bank Japan News

[2024-11-20]

224 new PDB entries have been released on 2024-11-20.

[2024-11-01]

A new portal integrating genome variants and protein structures has released

[2024-10-30]

[wwPDB] Deprecation of FTP File Download Protocol on November 1, 2024

[2024-10-25]

PDBj luncheon seminar at APBJC 2024

[2024-10-17]

[wwPDB] The Nobel Prize in Chemistry 2024