EMPIAR-11242 - Movies from 30S-RbfA-RimP-RsmA dataset [4395 multi-frame micrographs composed of 27 frames each in MRCS format]

EMPIAR-11242

Movies from 30S-RbfA-RimP-RsmA dataset [4395 multi-frame micrographs composed of 27 frames each in MRCS format]

Publication:

A conserved rRNA switch is central to decoding site maturation on the small ribosomal subunit.

Schedlbauer A, Iturrioz I, Ochoa-Lizzarralde B, Diercks T, López-Alonso JP, Lavin JL, Kaminishi T, Çapuni R, Dhimole N, De Astigarraga E, Gil-Garton D, Fucini P, Connell SR

Science advances 7 (2021) eabf7547-eabf7547

PMID: 34088665

DOI: 10.1126/sciadv.abf7547

Related EMDB entry:

EMD-11772 EMD-11771

Deposited:

2020-06-17

Released:

2022-11-15

Last modified:

2022-11-15

Imageset size:

3.62 TB

Imageset DOI:

10.6019/EMPIAR-11242

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multiframe movies of E. coli 30S subunit bound by RbfA,RimP, and RsmA

Category:

micrographs - multiframe

Image format:

MRCS

No. of images or tilt series:

4395

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.085, 1.085)

Details:

Falcon III (linear) movies collected at eBIC - Titan Krios (M03, em17171-12) at 300 kV, 1.085 Å/pixel and a total exposure of 42 e-/Å^2

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Citations

AlignPCA-2D: PCA-reduced Euclidean vector alignment for 2D classification in cryo-EM.

Ramírez-Aportela E, Zarrabeitia OL, Fonseca YC, Ceska T, Subramaniam S, Carazo JM, Sorzano COS. (2026)

A variational framework with composite sparse regularization for cryo-electron tomography reconstruction.

Yu C, Xu Z, Zeng Q, Wan X, El-Messiry H, Zhang F, Han R. (2026)

Helical reconstruction of amyloids in cryoSPARC.

Schaefer JH, O'Neill RT, Donnelly JP, Powers ET, Kelly JW, Lander GC. (2026)

An unattended image-processing pipeline for on-the-fly quality assessment and 3D exploration in cryo-EM.

Marchán Torres D, Conesa P, Garcia A, Iceta M, Broche L, Gragera M, Linares R, Kwong HS, Chichón FJ, Svensson O, Arranz R, Effantin G, Kandiah E, Carazo JM, Sorzano COS. (2026)

CryoFSL: an annotation-efficient, few-shot learning framework for robust protein particle picking in cryo-electron microscopy micrographs.

Poudel B, Gyawali R, Dhakal A, Cheng J, Xu D. (2026)

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.

Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

CryoWriter: a robotic solution for improved Cryo-EM grid preparation.

Kv C, Ekundayo B, Di Fabrizio M, Mohammed I, Radecke J, Stahlberg H, Kube M. (2026)