EMPIAR-11242 - Movies from 30S-RbfA-RimP-RsmA dataset [4395 multi-frame micrographs composed of 27 frames each in MRCS format]

EMPIAR-11242

Movies from 30S-RbfA-RimP-RsmA dataset [4395 multi-frame micrographs composed of 27 frames each in MRCS format]

Publication:

A conserved rRNA switch is central to decoding site maturation on the small ribosomal subunit.

Schedlbauer A, Iturrioz I, Ochoa-Lizzarralde B, Diercks T, López-Alonso JP, Lavin JL, Kaminishi T, Çapuni R, Dhimole N, De Astigarraga E, Gil-Garton D, Fucini P, Connell SR

Science advances 7 (2021) eabf7547-eabf7547

PMID: 34088665

DOI: 10.1126/sciadv.abf7547

Related EMDB entry:

EMD-11772 EMD-11771

Deposited:

2020-06-17

Released:

2022-11-15

Last modified:

2022-11-15

Imageset size:

3.98 TB

Imageset DOI:

10.6019/EMPIAR-11242

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multiframe movies of E. coli 30S subunit bound by RbfA,RimP, and RsmA

Category:

micrographs - multiframe

Image format:

MRCS

No. of images or tilt series:

4395

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.085, 1.085)

Details:

Falcon III (linear) movies collected at eBIC - Titan Krios (M03, em17171-12) at 300 kV, 1.085 Å/pixel and a total exposure of 42 e-/Å^2

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Citations

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Kim K, Li H, Clarke OB. (2025)

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CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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