EMPIAR-11129 - Cryo-EM of ADP-Pi-F-actin [multiple data sets in TIFF and MRCS formats]

EMPIAR-11129

Cryo-EM of ADP-Pi-F-actin [multiple data sets in TIFF and MRCS formats]

Publication:

Bending forces and nucleotide state jointly regulate F-actin structure

Reynolds MJ, Hachicho C, Carl AG, Gong R, Alushin GM

Nature 611 (2022) 380-386

PMID: 36289330

DOI: 10.1038/s41586-022-05366-w

Related EMDB entry:

EMD-27117 EMD-27115

Deposited:

2022-07-29

Released:

2022-09-13

Last modified:

2022-11-11

Imageset size:

2.53 TB

Imageset DOI:

10.6019/EMPIAR-11129

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
picked particles - single frame - processed

Image Sets

1. Unaligned multi-frame micrographs of ADP-F-actin

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4834

Image size:

(7420, 7676)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.515, 0.515)

Details:

This directory also contains motion-correction that was done on-the-fly. Re-running motion-correction locally is recommended.

Files:

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2. Polished single-frame particles of bent ADP-Pi-F-actin segments

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

14991

Image size:

(512, 512)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.03, 1.03)

Details:

This stack of particles was generated from the final Refine3D of the asymmetric, bent ADP-Pi-F-actin at EMD-27117 and PDB: 8D16 using the relion_stack_create command.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Polished single-frame particles of helical ADP-Pi-F-actin segments, for high-resolution

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

374778

Image size:

(512, 512)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.03, 1.03)

Details:

This set of particles was used for the final Refine3D of the helically-symmetric ADP-Pi-F-actin at EMD-27115 and PDB: 8D14
The run_data.star file corresponds to the final Refine3D that was run using the polished particle stacks in the Polish directory.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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