EMPIAR-11056 - Structure of bile acid transporter NTCP [multiple data sets in TIFF format]

EMPIAR-11056

Structure of bile acid transporter NTCP [multiple data sets in TIFF format]

Publication:

Structure of bile acid transporter NTCP crucial for hepatitis B virus entry

Asami J, Kimura KT, Fujita-Fujiharu Y, Ishida H, Zhang Z, Nomura Y, Liu K, Uemura T, Sato Y, Ono M, Yamamoto M, Noda T, Shigematsu H, Drew D, Iwata S, Shimizu T, Nomura N, Ohto U

Nature (2022)

PMID: 35580629

DOI: 10.1038/s41586-022-04845-4

Related EMDB entry:

EMD-31840 EMD-32759 EMD-31839 EMD-31838 EMD-31837

Deposited:

2022-05-18

Released:

2022-06-13

Last modified:

2022-06-13

Imageset size:

8.62 TB

Imageset DOI:

10.6019/EMPIAR-11056

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. Raw movies for Human NTCP (Q261A) - YN69202Fab (EMD-31837, PDB 7VAD)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5434

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

HT: 300 kV
Cs: 2.7 mm
Flux: 0.95 e/Å2/sec

Please use CountCDSRef_ja_115-1_0007_May14_21.29.53.mrc as a gain reference without rotation and flipping.

Four digits in the file names (0000 to 0008) represent beam shift directions.

Files:

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2. Raw movies for Bovine NTCP (Q261A) - YN69202Fab (EMD-31838, PDB 7VAE)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2712

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

HT: 300 kV
Cs: 2.7 mm
Flux: 0.95 e/Å2/sec

Please use CountCDSRef_ja_92-1_0000_Apr28_20.18.32.mrc as a gain reference without rotation and flipping.

Four digits in the file names (0000 to 0008) represent beam shift directions.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Raw movies for Rat NTCP (Q261A) - YN69202Fab (EMD-31839, PDB 7VAE)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

9932

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

HT: 300 kV
Cs: 2.7 mm
Flux: 0.95 e/Å2/sec

This structure was solved from datasets collected in three sessions.

Please use CountCDSRef_ja_73-1_0000_Apr15_19.29.35.mrc (for 20210415_shimizulab_210414_2_P2_JA), CountCDSRef_ja_119-1_0000_Apr29_11.04.43.mrc (for 20210429_shimizulab_210427_1_H1_JA) and CountCDSRef_ja_111-1_0000_Apr30_22.44.30.mrc (for 20210430_shimizulab_210430_9_E3_JA) as a gain reference without rotation and flipping.

Four digits in the file names (0000 to 0008) represent beam shift directions.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. Raw movies for Human NTCP (Q261A) - YN69202Fab-myr-preS1 (EMD-31840, PDB 7VAF)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7496

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

HT: 300 kV
Cs: 2.7 mm
Flux: 0.95 e/Å2/sec

This structure was solved from datasets collected in two sessions.

Please use CountCDSRef_ja_147-1_0000_Jul22_19.18.51.mrc (for 20210722_shimizulab_210720_4_F4_JA) and CountCDSRef_ja_159-1_0000_Aug06_15.07.36.mrc (for 20210806_shimizulab_210804_1_B1_JA) as a gain reference without rotation and flipping.

Four digits in the file names (0000 to 0008) represent beam shift directions.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

5. Raw movies for Human NTCP (wildtype) - YN69202Fab (EMD-32759, PDB 7WSI)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3411

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

HT: 300 kV
Cs: 2.7 mm
Flux: 0.95 e/Å2/sec

Please use CountCDSRef_ja_125-1_0000_Dec18_16.34.47.mrc as a gain reference without rotation and flipping.

Four digits in the file names (0000 to 0008) represent beam shift directions.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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