EMPIAR-11053 - Structure of pre-60S particle bound to DRG1(AFG2) [multiple data sets in TIFF format]

EMPIAR-11053

Structure of pre-60S particle bound to DRG1(AFG2) [multiple data sets in TIFF format]

Publication:

Visualizing maturation factor extraction from the nascent ribosome by the AAA-ATPase Drg1

Prattes M, Grishkovskaya I, Hodirnau VV, Hetzmannseder C, Zisser G, Sailer C, Kargas V, Loibl M, Gerhalter M, Kofler L, Warren AJ, Stengel F, Haselbach D, Bergler H

Nature structural & molecular biology (2022)

PMID: 36097293

DOI: 10.1038/s41594-022-00832-5

Related EMDB entry:

EMD-14437 EMD-14471

Deposited:

2022-04-08

Released:

2022-09-23

Last modified:

2022-09-23

Imageset size:

3.85 TB

Imageset DOI:

10.6019/EMPIAR-11053

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. DRG1pre60Sparticle

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7947

Image size:

(5760, 4092)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.07, 1.07)

Details:

Picked particles and other parameters can be found in the .cs file

Files:

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2. DRG1pre60Sparticle

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4385

Image size:

(2836809, 5972114)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.07, 1.07)

Details:

Additional data collection of tilted series for Bud20_DRG1 as presence of preferred orientation in the 1st data set. Picked particles and other parameters can be found in the .cs file

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. DRG1_substrate

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7947

Image size:

(2836809, 6382304)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.07, 1.07)

Details:

Picked particles file for DRG1 alone

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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