EMPIAR-11053 - Structure of pre-60S particle bound to DRG1(AFG2) [multiple data sets in TIFF format]

EMPIAR-11053

Structure of pre-60S particle bound to DRG1(AFG2) [multiple data sets in TIFF format]

Publication:

Visualizing maturation factor extraction from the nascent ribosome by the AAA-ATPase Drg1

Prattes M, Grishkovskaya I, Hodirnau VV, Hetzmannseder C, Zisser G, Sailer C, Kargas V, Loibl M, Gerhalter M, Kofler L, Warren AJ, Stengel F, Haselbach D, Bergler H

Nature structural & molecular biology (2022)

PMID: 36097293

DOI: 10.1038/s41594-022-00832-5

Related EMDB entry:

EMD-14437 EMD-14471

Deposited:

2022-04-08

Released:

2022-09-23

Last modified:

2022-09-23

Imageset size:

3.85 TB

Imageset DOI:

10.6019/EMPIAR-11053

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. DRG1pre60Sparticle

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7947

Image size:

(5760, 4092)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.07, 1.07)

Details:

Picked particles and other parameters can be found in the .cs file

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. DRG1pre60Sparticle

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4385

Image size:

(2836809, 5972114)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.07, 1.07)

Details:

Additional data collection of tilted series for Bud20_DRG1 as presence of preferred orientation in the 1st data set. Picked particles and other parameters can be found in the .cs file

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. DRG1_substrate

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7947

Image size:

(2836809, 6382304)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.07, 1.07)

Details:

Picked particles file for DRG1 alone

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Quick Links

Talks and Tutorials

EMPIAR Quick tour

Statistics

Publications

Re-use case study

EMPIAR in the news

Claim entries to your ORCiD

COVID-19 Data Portal

EMDB

PDBe

BioImage Archive

EMNavigator@PDBj

PDBj

RCSB@PDBj

EMDataResoruce@RCSB,EMDB,NCMI

Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

See all citations

Protein Data Bank Japan News

[2024-11-13]

236 new PDB entries have been released on 2024-11-13.

[2024-11-01]

A new portal integrating genome variants and protein structures has released

[2024-10-30]

[wwPDB] Deprecation of FTP File Download Protocol on November 1, 2024

[2024-10-25]

PDBj luncheon seminar at APBJC 2024

[2024-10-17]

[wwPDB] The Nobel Prize in Chemistry 2024