EMPIAR-11021 - Cryo-EM data used for the determination of the structures of LACV-L in 3 different states: replication initiation state, transcription capped primer active site entry state and transcription initiation state [14341 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-11021

Cryo-EM data used for the determination of the structures of LACV-L in 3 different states: replication initiation state, transcription capped primer active site entry state and transcription initiation state [14341 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Structural snapshots of La Crosse virus polymerase reveal the mechanisms underlying Peribunyaviridae replication and transcription

Arragain B, Durieux Trouilleton Q, Baudin F, Provaznik J, Azevedo N, Cusack S, Schoehn G, Malet H

Nature communications 13 (2022)

PMID: 35173159

DOI: 10.1038/s41467-022-28428-z

Related EMDB entry:

EMD-13040 EMD-13043 EMD-13041

Deposited:

2022-04-05

Released:

2022-06-20

Last modified:

2022-06-20

Imageset size:

2.38 TB

Imageset DOI:

10.6019/EMPIAR-11021

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multiframe micrographs of LACV-L incubated with capped RNA 14-mer, 3' vRNA 1-25, 5' 1-17BPm, UTP, ATP, MgCl2

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

14341

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.645, 0.645)

Details:

---

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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