EMPIAR-11005 - Conformational rearrangements upon start codon recognition in human 48S translation initiation complex [multiple data sets in MRC and MRCS formats]

EMPIAR-11005

Conformational rearrangements upon start codon recognition in human 48S translation initiation complex [multiple data sets in MRC and MRCS formats]

Publication:

Conformational rearrangements upon start codon recognition in human 48S translation initiation complex.

Yi SH, Petrychenko V, Schliep JE, Goyal A, Linden A, Chari A, Urlaub H, Stark H, Rodnina MV, Adio S, Fischer N

Nucleic acids research 50 (2022) 5282-5298

PMID: 35489072

DOI: 10.1093/nar/gkac283

Related EMDB entry:

EMD-14114 EMD-14113

Deposited:

2022-04-07

Released:

2022-05-17

Last modified:

2022-05-23

Imageset size:

1.15 TB

Imageset DOI:

10.6019/EMPIAR-11005

Experimental metadata:

Download xml json

Contains:

micrographs - single frame
picked particles - single frame - processed

Image Sets

1. Motion-corrected, dose-weighted micrographs

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

15544

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.16, 1.16)

Details:

Micrographs were obtained from movie-stacks of 20 frames à 2.4 e/A^2 by global motion-correction and dose-weighting using MotionCor2, CTF parameters were estimated using GCTF 1.06 and particles were selected by GAUTOMATCH.
- Micrographs .star-file: "data/micrographs_gctf.star"
- Particle coordinates: "*data/micrographs/*_automatch.star"
- 3D masks used for sorting particles in 180px box at 2.32Apix: "data/mask180px_40Shead.mrc" & "data/mask180px_TCandDC.mrc"

Files:

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2. Particles of human 48S IC in open state ("open")

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

57148

Image size:

(360, 360)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.16, 1.16)

Details:

Particles .star-file & 3D mask for final 3D refinement of open state (EMD-14113): "data/particles_48SIC_open.star" & "data/mask360px_48SIC_open.mrc"

Files:

Archives and downloads the selected files into an uncompressed zip file.

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Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Particles of human 48S IC in closed state ("closed")

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

364950

Image size:

(360, 360)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.16, 1.16)

Details:

Particles .star-file & 3D mask for final 3D refinement of closed state (EMD-14114): "data/particles_48SIC_closed.star" & "data/mask360px_48SIC_closed.mrc"

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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